Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm.

Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm.

We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm. Enriched viral nucleic acid was converted to double...

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Bibliographic Details
Main Authors: Matthew Cotten, Bas Oude Munnink, Marta Canuti, Martin Deijs, Simon J Watson, Paul Kellam, Lia van der Hoek
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3973683?pdf=render

Internet

http://europepmc.org/articles/PMC3973683?pdf=render

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