Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus <em>Orthotrichum</em> Using a Small Throughput Pyrosequencing Machine

Isolation and Characterization of Simple Sequence Repeats (SSR) Markers from the Moss Genus <em>Orthotrichum</em> Using a Small Throughput Pyrosequencing Machine

Here, we report the results of next-generation sequencing on the GS Junior system to identify a large number of microsatellites from the epiphytic moss <em>Orthotrichum speciosum</em>. Using a combination of a total (non-enrichment) genomic library and small-scale 454 pyr...

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Bibliographic Details
Main Authors: Vítězslav Plášek, Mirosław Kwaśniewski, Monika Szczecińska, Karolina Chwiałkowska, Monika Milewicz, Jakub Sawicki
Format: Article
Language:English
Published: MDPI AG 2012-06-01
Series:International Journal of Molecular Sciences
Subjects:
SSR markers
<em>Orthotrichum</em>
454 pyrosequencing
Online Access:http://www.mdpi.com/1422-0067/13/6/7586

Internet

http://www.mdpi.com/1422-0067/13/6/7586

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