Construction and comprehensive characterization of an EcLDCc-CatIB set—varying linkers and aggregation inducing tags

Construction and comprehensive characterization of an EcLDCc-CatIB set—varying linkers and aggregation inducing tags

Abstract Background In recent years, the production of inclusion bodies that retained substantial catalytic activity was demonstrated. These catalytically active inclusion bodies (CatIBs) were formed by genetic fusion of an aggregation inducing tag to a gene of interest via short linker polypeptides...

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Bibliographic Details
Main Authors: Kira Küsters, Martina Pohl, Ulrich Krauss, Gizem Ölçücü, Sandor Albert, Karl-Erich Jaeger, Wolfgang Wiechert, Marco Oldiges
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Microbial Cell Factories
Subjects:
Catalytically active inclusion bodies
Immobilization
Protein aggregates
Protein engineering
Downstream processing
Microscopic analysis
Online Access:https://doi.org/10.1186/s12934-021-01539-w

Internet

https://doi.org/10.1186/s12934-021-01539-w

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