A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information

A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information

Abstract Background Subunits of ribosomal RNA genes (rDNAs) characterized by PCR-based protocols have been the proxy for studies in microbial taxonomy, phylogenetics, evolution and ecology. However, relevant factors have shown to interfere in the experimental outputs in a variety of systems. In this...

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Bibliographic Details
Main Authors: Hellen Ribeiro Martins dos Santos, Caio Suzart Argolo, Ronaldo Costa Argôlo-Filho, Leandro Lopes Loguercio
Format: Article
Language:English
Published: BMC 2019-04-01
Series:BMC Microbiology
Subjects:
Microbial ecology and diversity
Community structure
Plant-associated bacteria
16S rDNA metagenomics
Fingerprinting methods
PCR-RFLP
Online Access:http://link.springer.com/article/10.1186/s12866-019-1446-2

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http://link.springer.com/article/10.1186/s12866-019-1446-2

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