Extracellular vesicle separation from milk and infant milk formula using acid precipitation and ultracentrifugation

Summary: Separation of highly enriched extracellular vesicles (EVs) fractions from milk is desirable for quantification, cargo analysis, functional characterization, and investigation as delivery vehicles for nutrients and/or therapeutics. However, a rigorous, reproducible protocol is lacking. This...

Full description

Bibliographic Details
Main Authors: Anindya Mukhopadhya, Jessie Santoro, Lorraine O’Driscoll
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100527X