Generation, maintenance, and monitoring of gnotobiotic mice

Summary: Gnotobiology has revolutionized the study of microbiota-host interactions. This protocol explains how to generate, maintain, and monitor gnotobiotic mice. Three monitoring methods are presented and compared: bacterial culture, microscopy to visualize the presence (or absence) of bacteria us...

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Bibliographic Details
Main Authors: Amanda Z. Zucoloto, Ian-Ling Yu, Kathy D. McCoy, Braedon McDonald
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002434