Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors

Summary: The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax...

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Bibliographic Details
Main Authors: Tingting Zhang, Tengyuan Liu, Bassem A. Hassan
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004664