Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome.

A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 20...

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Bibliographic Details
Main Authors: Benjamin Lopez-Jimena, Stefanie Wehner, Graham Harold, Mohammed Bakheit, Sieghard Frischmann, Michaël Bekaert, Oumar Faye, Amadou Alpha Sall, Manfred Weidmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-05-01
Series:PLoS Neglected Tropical Diseases
Online Access:http://europepmc.org/articles/PMC5973553?pdf=render