Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa

Abstract A gene named zenc, encoding a zearalenone lactonase from Neurospora crassa, was over-expressed in Pichia pastoris. The zenc gene is 888-bp in length, encoding a 295-residue polypeptide. Purified ZENC has maximal activity at pH 8.0 and 45 °C, and is highly stable at pH 6.0–8.0 for 1 h at 37 ...

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Bibliographic Details
Main Authors: Ke Bi, Wen Zhang, Zhizhuang Xiao, Dawei Zhang
Format: Article
Language:English
Published: SpringerOpen 2018-12-01
Series:AMB Express
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13568-018-0723-z