Characterization, expression and application of a zearalenone degrading enzyme from Neurospora crassa
Abstract A gene named zenc, encoding a zearalenone lactonase from Neurospora crassa, was over-expressed in Pichia pastoris. The zenc gene is 888-bp in length, encoding a 295-residue polypeptide. Purified ZENC has maximal activity at pH 8.0 and 45 °C, and is highly stable at pH 6.0–8.0 for 1 h at 37 ...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
SpringerOpen
2018-12-01
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Series: | AMB Express |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13568-018-0723-z |