EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM Evaluación del factor de crecimiento IGF-I en la maduración in vitro de ovocitos de perras en el medio TCM 199 AVALIAÇÃO DO FATOR DE CRESCIMENTO IGF-I NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS NO MEIO TCM 199

EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM Evaluación del factor de crecimiento IGF-I en la maduración in vitro de ovocitos de perras en el medio TCM 199 AVALIAÇÃO DO FATOR DE CRESCIMENTO IGF-I NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS NO MEIO TCM 199

The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris) and Hoeschst 33342 for nuclear and cytopla...

Full description

Bibliographic Details
Main Authors: Maricy Apparício Ferreira, Ana Paula Coelho Ribeiro, Marco Antônio Machado, Kellen de Sousa, Oliveira, Gilson Hélio Toniollo
Format: Article
Language:English
Published: Universidade Federal de Goiás 2009-07-01
Series:Ciência Animal Brasileira
Subjects:
Sanidade Animal
Online Access:http://www.revistas.ufg.br/index.php/vet/article/view/1121

Similar Items

Similar Items