EVALUATION OF THE GROWTH FACTOR IGF-I IN THE IN VITRO MATURATION OF CANINE OOCYTES IN THE TCM 199 MEDIUM Evaluación del factor de crecimiento IGF-I en la maduración in vitro de ovocitos de perras en el medio TCM 199 AVALIAÇÃO DO FATOR DE CRESCIMENTO IGF-I NA MATURAÇÃO IN VITRO DE OÓCITOS CANINOS NO MEIO TCM 199
The aim was to evaluate the supplementation of recombinant IGF-I growth factor at concentration of 100?g/mL in 500µL of TCM 199 medium in the in vitro maturation of canine oocytes. The oocytes were stained with isotiocianate of fluorescein (Lens cullinaris) and Hoeschst 33342 for nuclear and cytopla...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Universidade Federal de Goiás
2009-07-01
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Series: | Ciência Animal Brasileira |
Subjects: | |
Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/1121 |