An improved allele-specific PCR primer design method for SNP marker analysis and its application

An improved allele-specific PCR primer design method for SNP marker analysis and its application

<p>Abstract</p> <p>Background</p> <p>Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) still restricts its applicat...

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Bibliographic Details
Main Authors: Liu Jing, Huang Shunmou, Sun Meiyu, Liu Shengyi, Liu Yumei, Wang Wanxing, Zhang Xiurong, Wang Hanzhong, Hua Wei
Format: Article
Language:English
Published: BMC 2012-08-01
Series:Plant Methods
Subjects:
SNP
AS-PCR
Mismatch
Polymorphism
Destabilization
Online Access:http://www.plantmethods.com/content/8/1/34

Internet

http://www.plantmethods.com/content/8/1/34

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