Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-06-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01139/full |