Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants. Based on the Minimum Information for Publication of Real-Time Quantitative PCR Experiments (MIQE) guidelines, it is necessary to select and validate...

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Bibliographic Details
Main Authors: Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-06-01
Series:Frontiers in Plant Science
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.01139/full