Tandem CTCF sites function as insulators to balance spatial chromatin contacts and topological enhancer-promoter selection

Tandem CTCF sites function as insulators to balance spatial chromatin contacts and topological enhancer-promoter selection

Abstract Background CTCF is a key insulator-binding protein, and mammalian genomes contain numerous CTCF sites, many of which are organized in tandem. Results Using CRISPR DNA-fragment editing, in conjunction with chromosome conformation capture, we find that CTCF sites, if located between enhancers...

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Bibliographic Details
Main Authors: Zhilian Jia, Jingwei Li, Xiao Ge, Yonghu Wu, Ya Guo, Qiang Wu
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Genome Biology
Subjects:
CTCF
Insulator
Promoter/enhancer selection
3D genome
Gene regulation
Loop extrusion
Online Access:http://link.springer.com/article/10.1186/s13059-020-01984-7

Internet

http://link.springer.com/article/10.1186/s13059-020-01984-7

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