A Case Study into Microbial Genome Assembly Gap Sequences and Finishing Strategies

This study characterized regions of DNA which remained unassembled by either PacBio and Illumina sequencing technologies for seven bacterial genomes. Two genomes were manually finished using bioinformatics and PCR/Sanger sequencing approaches and regions not assembled by automated software were anal...

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Bibliographic Details
Main Authors: Sagar M. Utturkar, Dawn M. Klingeman, Richard A. Hurt, Steven D. Brown
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-07-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01272/full