Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes

Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes

Abstract Background Cryopreserved human peripheral blood mononuclear cells (PBMCs) are a commonly used sample type for a variety of immunological assays. Many factors can affect the quality of PBMCs, and careful consideration and validation of an appropriate PBMC isolation and cryopreservation metho...

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Bibliographic Details
Main Authors: Han Chen, Christian M. Schürch, Kevin Noble, Kenneth Kim, Peter O. Krutzik, Erika O’Donnell, Jason Vander Tuig, Garry P. Nolan, David R. McIlwain
Format: Article
Language:English
Published: BMC 2020-03-01
Series:BMC Immunology
Subjects:
Peripheral blood mononuclear cells
BD Cell Preparation Tubes
Lymphoprep Tubes
Flow cytometry
T cell
Cytokine
Online Access:http://link.springer.com/article/10.1186/s12865-020-00345-0

Internet

http://link.springer.com/article/10.1186/s12865-020-00345-0

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