Optimization of small RNA library preparation protocol from human urinary exosomes

Optimization of small RNA library preparation protocol from human urinary exosomes

Abstract Background Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library prep...

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Bibliographic Details
Main Authors: Dolores Olivares, Javier Perez-Hernandez, Daniel Perez-Gil, Felipe J. Chaves, Josep Redon, Raquel Cortes
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Journal of Translational Medicine
Subjects:
Exosome
microRNA
Urine
Adapter-dimer
Size selection step
Next generation sequencing
Online Access:http://link.springer.com/article/10.1186/s12967-020-02298-9

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http://link.springer.com/article/10.1186/s12967-020-02298-9

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