Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber–pumpkin grafted plants
Background Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput technique to measure mRNA transcript levels. The accuracy of this evaluation of gene expression depends on the use of optimal reference genes. Cucumber–pumpkin grafted plants, made by grafting a cucumber scion onto pu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2019-04-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/6536.pdf |