Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations

Abstract Background Plasmid propagation in recombination strains such as Escherichia coli DH5α is regarded as a beneficial instrument for stable amplification of the DNA materials. Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, t...

Full description

Bibliographic Details
Main Authors: Ahmed A. Hamed, Mohamed Khedr, Mohamed Abdelraof
Format: Article
Language:English
Published: SpringerOpen 2020-12-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
β-Galactosidase
Escherichia coli DH5α
Trans-conjugation
α-Complementation
Purification
Online Access:https://doi.org/10.1186/s43141-020-00096-w

Internet

https://doi.org/10.1186/s43141-020-00096-w

Similar Items