Microfluidic qPCR Enables High Throughput Quantification of Microbial Functional Genes but Requires Strict Curation of Primers

Microfluidic qPCR Enables High Throughput Quantification of Microbial Functional Genes but Requires Strict Curation of Primers

Quantification of microbial functional genes enhances predictions of soil biogeochemical process rates, but reliance on low-throughput quantitative PCR (qPCR) limits the scope of ecological studies to a handful of targets. Here, we explore whether microfluidic qPCR (MFQPCR) is a viable high-throughp...

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Bibliographic Details
Main Authors: Sally L. Crane, Josie van Dorst, Grant C. Hose, Catherine K. King, Belinda C. Ferrari
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-11-01
Series:Frontiers in Environmental Science
Subjects:
microfluidic qPCR
quantitative PCR
functional genes
nitrogen cycle
hydrocarbon degradation
microbial community
Online Access:https://www.frontiersin.org/article/10.3389/fenvs.2018.00145/full

Internet

https://www.frontiersin.org/article/10.3389/fenvs.2018.00145/full

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