Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization

Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization

Electrospray mass spectrometry is applied to determine apparent binding energies and quasi equilibrium dissociation constants of immune complex dissociation reactions in the gas phase. Myoglobin, a natural protein-ligand complex, has been used to develop the procedure which starts from determining m...

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Bibliographic Details
Main Authors: Bright D. Danquah, Kwabena F. M. Opuni, Claudia Roewer, Cornelia Koy, Michael O. Glocker
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Molecules
Subjects:
mass spectrometric epitope mapping
gas phase immune complex dissociation
apparent gas phase dissociation constants
apparent gas phase activation energies
ITEM-TWO
native mass spectrometry
Online Access:https://www.mdpi.com/1420-3049/25/20/4776

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https://www.mdpi.com/1420-3049/25/20/4776

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