Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology

Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology

Real-time molecular techniques have become the reference methods for direct diagnosis of pathogens. The reduction of steps is a key factor in order to decrease the risk of human errors resulting in invalid series and delayed results. We describe here a process of preparation of oligonucleotide prime...

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Bibliographic Details
Main Authors: Laurence Thirion, Audrey Dubot-Peres, Laura Pezzi, Iban Corcostegui, Mhammed Touinssi, Xavier de Lamballerie, Remi N. Charrel
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Viruses
Subjects:
freeze-drying
lyophilization
pcr
diagnosis
virus
pathogen
taqman
emerging
epidemic
Online Access:https://www.mdpi.com/1999-4915/12/2/159

Internet

https://www.mdpi.com/1999-4915/12/2/159

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