Analysis of Wheat Prolamins, the Causative Agents of Celiac Sprue, Using Reversed Phase High Performance Liquid Chromatography (RP-HPLC) and Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS)

Wheat prolamins, commonly known as “gluten”, are a complex mixture of 71–78 proteins, which constitute ~80% of the proteins in the wheat grains and supply 50% of the global dietary protein demand. Prolamins are also responsible for numerous gluten-induced disorders and determine the unique visco-e...

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Bibliographic Details
Main Authors: Jaime H. Mejías, Xiaoqiao Lu, Claudia Osorio, Jeffrey L. Ullman, Diter von Wettstein, Sachin Rustgi
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:Nutrients
Subjects:
Online Access:http://www.mdpi.com/2072-6643/6/4/1578