Standardization of Northern blot non-radioactive protocol used in the detection of small RNAs in Vero line cells

Standardization of Northern blot non-radioactive protocol used in the detection of small RNAs in Vero line cells

The interest in detection, identification and functional characterization of small non-coding RNAs (snRNAs), has generated the need to optimize the methodologies commonly used in its detection in specificity and sensitivity, The Quantitative reverse transcription PCR (RT-qPCR) and Northern blot. Eve...

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Bibliographic Details
Main Authors: Natalia Campillo Pedroza, Juan Pablo Franco Salazar, Juan Carlos Gallego Gómez
Format: Article
Language:Spanish
Published: Universidad Nacional de Colombia 2015-07-01
Series:Revista Colombiana de Biotecnología
Subjects:
biología molecular
técnicas moleculares
investigación
1-ethyl-3-(-3-dimethylaminopropyl) carboniimide)
Digoxigenine
Northern blot non-radioactive
LNA probe
sRNAs
Online Access:https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522

Internet

https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522

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