Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyra...
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2020-09-01
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Online Access: | https://doi.org/10.1038/s41467-020-18456-y |
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doaj-d57278de37904fdbafba88ac63c972982021-09-19T11:51:38ZengNature Publishing GroupNature Communications2041-17232020-09-0111111010.1038/s41467-020-18456-yMultiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precisionRohit Agarwal0Karl E. Duderstadt1Structure and Dynamics of Molecular Machines, Max Planck Institute of BiochemistryStructure and Dynamics of Molecular Machines, Max Planck Institute of BiochemistrySingle molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase.https://doi.org/10.1038/s41467-020-18456-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rohit Agarwal Karl E. Duderstadt |
spellingShingle |
Rohit Agarwal Karl E. Duderstadt Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision Nature Communications |
author_facet |
Rohit Agarwal Karl E. Duderstadt |
author_sort |
Rohit Agarwal |
title |
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
title_short |
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
title_full |
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
title_fullStr |
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
title_full_unstemmed |
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
title_sort |
multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2020-09-01 |
description |
Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase. |
url |
https://doi.org/10.1038/s41467-020-18456-y |
work_keys_str_mv |
AT rohitagarwal multiplexflowmagnetictweezersrevealrareenzymaticeventswithsinglemoleculeprecision AT karleduderstadt multiplexflowmagnetictweezersrevealrareenzymaticeventswithsinglemoleculeprecision |
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1717375452849373184 |