Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision

Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyra...

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Main Authors: Rohit Agarwal, Karl E. Duderstadt
Format: Article
Language:English
Published: Nature Publishing Group 2020-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18456-y
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spelling doaj-d57278de37904fdbafba88ac63c972982021-09-19T11:51:38ZengNature Publishing GroupNature Communications2041-17232020-09-0111111010.1038/s41467-020-18456-yMultiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precisionRohit Agarwal0Karl E. Duderstadt1Structure and Dynamics of Molecular Machines, Max Planck Institute of BiochemistryStructure and Dynamics of Molecular Machines, Max Planck Institute of BiochemistrySingle molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase.https://doi.org/10.1038/s41467-020-18456-y
collection DOAJ
language English
format Article
sources DOAJ
author Rohit Agarwal
Karl E. Duderstadt
spellingShingle Rohit Agarwal
Karl E. Duderstadt
Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
Nature Communications
author_facet Rohit Agarwal
Karl E. Duderstadt
author_sort Rohit Agarwal
title Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
title_short Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
title_full Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
title_fullStr Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
title_full_unstemmed Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
title_sort multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-09-01
description Single molecule force measurements have shed light on dynamic biological events, but rare events escape notice owing to low throughput of the methods. Here, the authors combine an array of magnetic tweezers with lateral flow to increase throughput 100-fold, and detect rare DNA breaks induced by gyrase.
url https://doi.org/10.1038/s41467-020-18456-y
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AT karleduderstadt multiplexflowmagnetictweezersrevealrareenzymaticeventswithsinglemoleculeprecision
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