Characterization of 16 novel microsatellite loci for Ephippiger diurnus (Orthoptera: Tettigoniidae) using pyrosequencing technology and cross-species amplification
A novel panel of 16 microsatellite markers, obtained by pyrosequencing of enriched genomic libraries, is reported for the flightless European bushcricket Ephippiger diurnus (Dufour) (Orthoptera: Tettigoniidae). Five multiplex and one simplex PCR protocols were optimized, and the polymorphism at the...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Institute of Entomology, Biology Centre, Czech Academy of Science
2016-04-01
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Series: | European Journal of Entomology |
Subjects: | |
Online Access: | https://www.eje.cz/artkey/eje-201601-0037_Characterization_of_16_novel_microsatellite_loci_for_Ephippiger_diurnus_Orthoptera_Tettigoniidae_using_pyros.php |