MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR
<p>Abstract</p> <p>Background</p> <p>Multiple approaches for the site-directed mutagenesis (SDM) have been developed. However, only several of them are designed for simultaneous introduction of multiple nucleotide alterations, and these are time consuming. In addition,...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2009-09-01
|
Series: | BMC Biotechnology |
Online Access: | http://www.biomedcentral.com/1472-6750/9/83 |