Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the...

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Bibliographic Details
Main Authors: Stephanie D. Cole, Aleksandr E. Miklos, Abel C. Chiao, Zachary Z. Sun, Matthew W. Lux
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
Cell-free systems
Cell-free expression
Cell-free extract
Synthetic biology
Methods
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X2030051X
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spelling doaj-d2ee50b9e5bf40fe8bb75cabadbd83492021-04-02T16:34:55ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2020-12-0154252267Methodologies for preparation of prokaryotic extracts for cell-free expression systemsStephanie D. Cole0Aleksandr E. Miklos1Abel C. Chiao2Zachary Z. Sun3Matthew W. Lux4US Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, Aberdeen Proving Ground, MD, 21010, USAUS Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, Aberdeen Proving Ground, MD, 21010, USADivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA; Synvitrobio Inc., San Francisco, CA, USADivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA; Synvitrobio Inc., San Francisco, CA, USAUS Army Combat Capabilities Development Command Chemical Biological Center, 8567 Ricketts Point Road, Aberdeen Proving Ground, MD, 21010, USA; Corresponding author. 8567 Ricketts Point Road, Bldg E3549 Room B140, APG, MD, 21010, USA.Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity of Escherichia coli genetic strains available and their corresponding utility. We then trace the history of cell-extract methodology over the past 20 years, showing key improvements that lower the entry level for new researchers. Next, we survey the rise of new prokaryotic cell-free systems, with associated methods, and the opportunities provided. Finally, we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.http://www.sciencedirect.com/science/article/pii/S2405805X2030051XCell-free systemsCell-free expressionCell-free extractSynthetic biologyMethods
collection DOAJ
language English
format Article
sources DOAJ
author Stephanie D. Cole
Aleksandr E. Miklos
Abel C. Chiao
Zachary Z. Sun
Matthew W. Lux
spellingShingle Stephanie D. Cole
Aleksandr E. Miklos
Abel C. Chiao
Zachary Z. Sun
Matthew W. Lux
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
Synthetic and Systems Biotechnology
Cell-free systems
Cell-free expression
Cell-free extract
Synthetic biology
Methods
author_facet Stephanie D. Cole
Aleksandr E. Miklos
Abel C. Chiao
Zachary Z. Sun
Matthew W. Lux
author_sort Stephanie D. Cole
title Methodologies for preparation of prokaryotic extracts for cell-free expression systems
title_short Methodologies for preparation of prokaryotic extracts for cell-free expression systems
title_full Methodologies for preparation of prokaryotic extracts for cell-free expression systems
title_fullStr Methodologies for preparation of prokaryotic extracts for cell-free expression systems
title_full_unstemmed Methodologies for preparation of prokaryotic extracts for cell-free expression systems
title_sort methodologies for preparation of prokaryotic extracts for cell-free expression systems
publisher KeAi Communications Co., Ltd.
series Synthetic and Systems Biotechnology
issn 2405-805X
publishDate 2020-12-01
description Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the diversity of Escherichia coli genetic strains available and their corresponding utility. We then trace the history of cell-extract methodology over the past 20 years, showing key improvements that lower the entry level for new researchers. Next, we survey the rise of new prokaryotic cell-free systems, with associated methods, and the opportunities provided. Finally, we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.
topic Cell-free systems
Cell-free expression
Cell-free extract
Synthetic biology
Methods
url http://www.sciencedirect.com/science/article/pii/S2405805X2030051X
work_keys_str_mv AT stephaniedcole methodologiesforpreparationofprokaryoticextractsforcellfreeexpressionsystems
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AT abelcchiao methodologiesforpreparationofprokaryoticextractsforcellfreeexpressionsystems
AT zacharyzsun methodologiesforpreparationofprokaryoticextractsforcellfreeexpressionsystems
AT matthewwlux methodologiesforpreparationofprokaryoticextractsforcellfreeexpressionsystems
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