Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Cell-free systems that mimic essential cell functions, such as gene expression, have dramatically expanded in recent years, both in terms of applications and widespread adoption. Here we provide a review of cell-extract methods, with a specific focus on prokaryotic systems. Firstly, we describe the...

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Bibliographic Details
Main Authors: Stephanie D. Cole, Aleksandr E. Miklos, Abel C. Chiao, Zachary Z. Sun, Matthew W. Lux
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X2030051X