Mutation Scanning in a Single and a Stacked Genetically Modified (GM) Event by Real-Time PCR and High Resolution Melting (HRM) Analysis

Mutation Scanning in a Single and a Stacked Genetically Modified (GM) Event by Real-Time PCR and High Resolution Melting (HRM) Analysis

Genetic mutations must be avoided during the production and use of seeds. In the European Union (EU), Directive 2001/18/EC requires any DNA construct introduced via transformation to be stable. Establishing genetic stability is critical for the approval of genetically modified organisms (GMOs). In...

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Bibliographic Details
Main Authors: Sina-Elisabeth Ben Ali, Zita Erika Madi, Rupert Hochegger, David Quist, Bernhard Prewein, Alexander G. Haslberger, Christian Brandes
Format: Article
Language:English
Published: MDPI AG 2014-10-01
Series:International Journal of Molecular Sciences
Subjects:
HRM analysis
Scorpion primer
MON810
MON88017 × MON810
GT73
genetic stability
stacked event
GMO
Online Access:http://www.mdpi.com/1422-0067/15/11/19898

Internet

http://www.mdpi.com/1422-0067/15/11/19898

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