Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts

Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts

Abstract Background In eco-epidemiological studies, Leishmania detection in vectors and reservoirs is frequently accomplished by high-throughput and sensitive molecular methods that target minicircle kinetoplast DNA (kDNA). A pan-Leishmania SYBR green quantitative PCR (qPCR) assay which detects the...

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Bibliographic Details
Main Authors: Myrthe Pareyn, Rik Hendrickx, Nigatu Girma, Sarah Hendrickx, Lieselotte Van Bockstal, Natalie Van Houtte, Simon Shibru, Louis Maes, Herwig Leirs, Guy Caljon
Format: Article
Language:English
Published: BMC 2020-06-01
Series:Parasites & Vectors
Subjects:
Spliced leader RNA
Kinetoplast DNA
Nucleic acid extraction
Sand fly
Reservoir
Real-time PCR
Online Access:http://link.springer.com/article/10.1186/s13071-020-04141-y

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http://link.springer.com/article/10.1186/s13071-020-04141-y

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