Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis
To study the role of invariant Natural Killer T cell (iNKT) cells in autoimmune thyroiditis, we derived two iNKT cell lines from the spleens of NOD·H2h4 mice, a strain that develops spontaneous autoimmune thyroiditis exacerbated by excess dietary iodine. The two lines were CD1d-restricted and expr...
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doaj-d2541c7934374be9b121dd9eaaff96682020-11-24T21:13:45ZengHindawi LimitedJournal of Thyroid Research2042-00722011-01-01201110.4061/2011/895923895923Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune ThyroiditisRajni B. Sharma0Xiaoguang Fan1Patrizio Caturegli2Noel R. Rose3C. Lynne Burek4Department of Pathology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Johns Hopkins University, Ross Research Building, 648, 720 Rutland Avenue, Baltimore, MD 21205, USADepartment of Pathology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Johns Hopkins University, Ross Research Building, 648, 720 Rutland Avenue, Baltimore, MD 21205, USADepartment of Pathology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Johns Hopkins University, Ross Research Building, 648, 720 Rutland Avenue, Baltimore, MD 21205, USADepartment of Pathology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Johns Hopkins University, Ross Research Building, 648, 720 Rutland Avenue, Baltimore, MD 21205, USADepartment of Pathology, School of Medicine, Bloomberg School of Public Health, Johns Hopkins Medical Institutions, Johns Hopkins University, Ross Research Building, 648, 720 Rutland Avenue, Baltimore, MD 21205, USATo study the role of invariant Natural Killer T cell (iNKT) cells in autoimmune thyroiditis, we derived two iNKT cell lines from the spleens of NOD·H2h4 mice, a strain that develops spontaneous autoimmune thyroiditis exacerbated by excess dietary iodine. The two lines were CD1d-restricted and expressed CD4+, DX5+, and the Vα4Jα281 gene segment, of the T-cell receptor α locus. Upon stimulation with α-galactosyl-ceramide (α-GalCer), both lines rapidly produced IL-2, IL-4, IFN-γ, IL-10, and TNF-α. Strikingly, a similar cytokine response was also induced by thyroglobulin, one of the most abundant protein in the thyroid gland and a major autoantigen in human autoimmune thyroiditis. Transfer of the iNKT cell lines to syngeneic hosts enhanced autoimmune thyroiditis. Intraperitoneal injections of α-GalCer in iodine primed mice also induced thyroid disease. This paper reports for the first time that iNKT cells respond to thyroglobulin and enhance autoimmune thyroiditis in iodine fed NOD·H2h4 mice.http://dx.doi.org/10.4061/2011/895923 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rajni B. Sharma Xiaoguang Fan Patrizio Caturegli Noel R. Rose C. Lynne Burek |
spellingShingle |
Rajni B. Sharma Xiaoguang Fan Patrizio Caturegli Noel R. Rose C. Lynne Burek Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis Journal of Thyroid Research |
author_facet |
Rajni B. Sharma Xiaoguang Fan Patrizio Caturegli Noel R. Rose C. Lynne Burek |
author_sort |
Rajni B. Sharma |
title |
Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis |
title_short |
Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis |
title_full |
Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis |
title_fullStr |
Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis |
title_full_unstemmed |
Invariant NKT Cell Lines Derived from the NOD·H2h4 Mouse Enhance Autoimmune Thyroiditis |
title_sort |
invariant nkt cell lines derived from the nod·h2h4 mouse enhance autoimmune thyroiditis |
publisher |
Hindawi Limited |
series |
Journal of Thyroid Research |
issn |
2042-0072 |
publishDate |
2011-01-01 |
description |
To study the role of invariant Natural Killer T cell (iNKT) cells in autoimmune thyroiditis, we derived two iNKT cell lines from the spleens of NOD·H2h4 mice, a strain that develops spontaneous autoimmune thyroiditis exacerbated by excess dietary iodine. The two lines were CD1d-restricted and expressed CD4+, DX5+, and the Vα4Jα281 gene segment, of the T-cell receptor α locus. Upon stimulation with α-galactosyl-ceramide (α-GalCer), both lines rapidly produced IL-2, IL-4, IFN-γ, IL-10, and TNF-α. Strikingly, a similar cytokine response was also induced by thyroglobulin, one of the most abundant protein in the thyroid gland and a major autoantigen in human autoimmune thyroiditis. Transfer of the iNKT cell lines to syngeneic hosts enhanced autoimmune thyroiditis. Intraperitoneal injections of α-GalCer in iodine primed mice also induced thyroid disease. This paper reports for the first time that iNKT cells respond to thyroglobulin and enhance autoimmune thyroiditis in iodine fed NOD·H2h4 mice. |
url |
http://dx.doi.org/10.4061/2011/895923 |
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