Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

Abstract Background Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stea...

Full description

Bibliographic Details
Main Authors: Dongbang Yao, Lingqia Su, Na Li, Jing Wu
Format: Article
Language:English
Published: BMC 2019-04-01
Series:Microbial Cell Factories
Subjects:
Alpha-amylase
Signal peptide
Chaperone
Mutant
High-cell-density fermentation
Online Access:http://link.springer.com/article/10.1186/s12934-019-1119-8

Internet

http://link.springer.com/article/10.1186/s12934-019-1119-8

Similar Items