Cell-based assay of MGAT2-driven diacylglycerol synthesis for profiling inhibitors: use of a stable isotope-labeled substrate and high-resolution LC/MS[S]

Cell-based assay of MGAT2-driven diacylglycerol synthesis for profiling inhibitors: use of a stable isotope-labeled substrate and high-resolution LC/MS[S]

To demonstrate monoacylglycerol acyltransferase 2 (MGAT2)-mediated enzyme activity in a cellular context, cells of the murine secretin tumor cell-1 line of enteroendocrine origin were used to construct human MGAT2-expressing recombinant cell lines. Low throughput and utilization of radiolabeled subs...

Full description

Bibliographic Details
Main Authors: Joelle M. Onorato, Ching-Hsuen Chu, Zhengping Ma, Lisa M. Kopcho, Hannguang J. Chao, R.Michael Lawrence, Dong Cheng
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Journal of Lipid Research
Subjects:
monoacylglycerol acyltransferase
diacylglycerol acyltransferase
triglycerides
liquid chromatography/mass spectrometry
cell-based assay
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520356029

Internet

http://www.sciencedirect.com/science/article/pii/S0022227520356029

Similar Items