Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia

Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% resp...

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Bibliographic Details
Main Author: Baghdad Science Journal
Format: Article
Language:Arabic
Published: College of Science for Women, University of Baghdad 2009-03-01
Series:Baghdad Science Journal
Subjects:
Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/956