A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hybridum, describing gRNA design, a transient protop...

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Bibliographic Details
Main Authors: Karolina Hus, Alexander Betekhtin, Artur Pinski, Magdalena Rojek-Jelonek, Ewa Grzebelus, Candida Nibau, Mingjun Gao, Katja E. Jaeger, Glyn Jenkins, John H. Doonan, Robert Hasterok
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9 system
targeted mutagenesis
Brachypodium distachyon
Brachypodium hybridum
Agrobacterium-mediated transformation
transient protoplast assay
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.00614/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2020.00614/full

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