Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.
Main Authors: | , , , , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-10-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-017-00847-3 |