Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Abstract Background Creating designer molecules using a combination of select domains from polyketide synthases and/or nonribosomal peptide synthetases (NRPS) continues to be a synthetic goal. However, an incomplete understanding of how protein-protein interactions and dynamics affect each of the do...

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Bibliographic Details
Main Authors: Fuchao Xu, Russell Butler, Kyle May, Megi Rexhepaj, Dayu Yu, Jiachen Zi, Yi Chen, Yonghong Liang, Jia Zeng, Joan Hevel, Jixun Zhan
Format: Article
Language:English
Published: BMC 2019-07-01
Series:Journal of Biological Engineering
Subjects:
Nonribosomal peptide synthetase
Methyltransferase domain
Substrate specificity
Domain removal
Heterologous expression
In vitro reaction
Online Access:http://link.springer.com/article/10.1186/s13036-019-0195-y

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http://link.springer.com/article/10.1186/s13036-019-0195-y

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