Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Abstract Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebraf...

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Bibliographic Details
Main Authors: Xiao Chen, Mi Liu, Hongyan Lou, Yiyi Lu, Meng-Tao Zhou, Rongying Ou, Yunsheng Xu, Kai-Fu Tang
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Genome Biology
Subjects:
TRIM21
Trim-away
Protein degradation
Knockdown
Online Access:http://link.springer.com/article/10.1186/s13059-019-1624-4
Description
Summary:Abstract Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.
ISSN:1474-760X

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