Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology

Abstract Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebraf...

Full description

Bibliographic Details
Main Authors: Xiao Chen, Mi Liu, Hongyan Lou, Yiyi Lu, Meng-Tao Zhou, Rongying Ou, Yunsheng Xu, Kai-Fu Tang
Format: Article
Language:English
Published: BMC 2019-01-01
Series:Genome Biology
Subjects:
TRIM21
Trim-away
Protein degradation
Knockdown
Online Access:http://link.springer.com/article/10.1186/s13059-019-1624-4

Internet

http://link.springer.com/article/10.1186/s13059-019-1624-4

Similar Items