Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy

Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy

This paper describes an unsupervised algorithm, which segments the nuclear envelope of HeLa cells imaged by Serial Block Face Scanning Electron Microscopy. The algorithm exploits the variations of pixel intensity in different cellular regions by calculating edges, which are then used to generate sup...

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Bibliographic Details
Main Authors: Cefa Karabağ, Martin L. Jones, Christopher J. Peddie, Anne E. Weston, Lucy M. Collinson, Constantino Carlos Reyes-Aldasoro
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Journal of Imaging
Subjects:
segmentation
HeLa cells
active contours
Hausdorff distance
Jaccard index
Online Access:https://www.mdpi.com/2313-433X/5/9/75

Internet

https://www.mdpi.com/2313-433X/5/9/75

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