A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms

Abstract Background Several fungi-specific primers target the 18S rRNA gene sequence, one of the prominent markers for fungal classification. The design of most primers goes back to the last decades. Since then, the number of sequences in public databases increased leading to the discovery of new fu...

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Bibliographic Details
Main Authors: Stefanos Banos, Guillaume Lentendu, Anna Kopf, Tesfaye Wubet, Frank Oliver Glöckner, Marlis Reich
Format: Article
Language:English
Published: BMC 2018-11-01
Series:BMC Microbiology
Subjects:
Fungi
18S rRNA gene sequence (SSU) primer
Annealing blocking oligonucleotides
Co-amplification
Real-time Q-PCR
Fungal biodiversity
Online Access:http://link.springer.com/article/10.1186/s12866-018-1331-4

Internet

http://link.springer.com/article/10.1186/s12866-018-1331-4

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