Astigmatic traction force microscopy (aTFM)

Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.

Bibliographic Details
Main Authors: Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary, Dong Li, Marco Fritzsche
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-22376-w