Ratio determination of plasma wild-type and L159R apoA-I using mass spectrometry: tools for studying apoA-IFin

Ratio determination of plasma wild-type and L159R apoA-I using mass spectrometry: tools for studying apoA-IFin

In this report, methods are described to isolate milligram quantities of a mutant apolipoprotein A-I (apoA-I) protein for use in structure-function studies. Expression of the L159R apoA-I mutation in humans reduces the concentration of plasma wild-type apoA-I, thus displaying a dominant negative phe...

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Bibliographic Details
Main Authors: John S. Owen, Manish S. Bharadwaj, Michael J. Thomas, Shaila Bhat, Michael P. Samuel, Mary G. Sorci-Thomas
Format: Article
Language:English
Published: Elsevier 2007-01-01
Series:Journal of Lipid Research
Subjects:
apolipoprotein A-I
protein expression
inclusion bodies
apolipoprotein A-I mutant
high-performance liquid chromatography-electrospray mass spectrometry
high-performance liquid chromatography-electrospray tandem mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520436545

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http://www.sciencedirect.com/science/article/pii/S0022227520436545

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