In vitro synthesis of gene-length single-stranded DNA
Abstract Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum length attainable, ranging from a few hundred nucleotides for chemical synthesis to a few thousand nucleotides for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-24677-5 |