Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress
Anxiety disorder is a well-recognized condition observed in subjects submitted to acute stress. Although the brain mechanisms underlying this disorder remain unclear, the available evidence indicates that oxidative stress and GABAergic dysfunction mediate the generation of stress-induced anxiety. Ca...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2020.598812/full |
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doaj-cb1b071cc0f34e2a87078f2274f592252021-01-18T05:00:25ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532021-01-011410.3389/fnbeh.2020.598812598812Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint StressWaldo Lucas Luz0Mateus Santos-Silva1Patrick Bruno Cardoso2Nadyme Assad3Edinaldo Rogério da Silva Moraes4Alan Barroso Araújo Grisólia5Danielle Valente Braga6Luana Ketlen Reis Leão7Suellen Alessandra Soares de Moraes8Adelaide da Conceição Passos9Evander de Jesus Oliveira Batista10Evander de Jesus Oliveira Batista11Amauri Gouveia12Karen R. H. Matos Oliveira13Anderson Manoel Herculano14Laboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Protozoology, Tropical Medicine Center, Federal University of Pará, Belém, BrazilLaboratory of Neuroscience and Behavior, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilLaboratory of Experimental Neuropharmacology, Institute of Biological Sciences, Federal University of Pará, Belém, BrazilAnxiety disorder is a well-recognized condition observed in subjects submitted to acute stress. Although the brain mechanisms underlying this disorder remain unclear, the available evidence indicates that oxidative stress and GABAergic dysfunction mediate the generation of stress-induced anxiety. Cannabinoids are known to be efficient modulators of behavior, given that the activation of the cannabinoid receptors type-1 (CB1 receptors) induces anxiolytic-like effects in animal models. In the present study, we aimed to describe the effects of the stimulation of the CB1 receptors on anxiety-like behavior, oxidative stress, and the GABA content of the brains of zebrafish submitted to acute restraint stress (ARS). The animals submitted to the ARS protocol presented evident anxiety-like behavior with increased lipid peroxidation in the brain tissue. The evaluation of the levels of GABA in the zebrafish telencephalon presented decreased levels of GABA in the ARS group in comparison with the control. Treatment with ACEA, a specific CB1 receptor agonist, prevented ARS-induced anxiety-like behavior and oxidative stress in the zebrafish brain. ACEA treatment also prevented a decrease in GABA in the telencephalon of the animals submitted to the ARS protocol. Overall, these preclinical data strongly suggest that the CB1 receptors represent a potential target for the development of the treatment of anxiety disorders elicited by acute stress.https://www.frontiersin.org/articles/10.3389/fnbeh.2020.598812/fullanxietyacute stressCB1 receptorsoxidative stressGABAzebrafish |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Waldo Lucas Luz Mateus Santos-Silva Patrick Bruno Cardoso Nadyme Assad Edinaldo Rogério da Silva Moraes Alan Barroso Araújo Grisólia Danielle Valente Braga Luana Ketlen Reis Leão Suellen Alessandra Soares de Moraes Adelaide da Conceição Passos Evander de Jesus Oliveira Batista Evander de Jesus Oliveira Batista Amauri Gouveia Karen R. H. Matos Oliveira Anderson Manoel Herculano |
| spellingShingle |
Waldo Lucas Luz Mateus Santos-Silva Patrick Bruno Cardoso Nadyme Assad Edinaldo Rogério da Silva Moraes Alan Barroso Araújo Grisólia Danielle Valente Braga Luana Ketlen Reis Leão Suellen Alessandra Soares de Moraes Adelaide da Conceição Passos Evander de Jesus Oliveira Batista Evander de Jesus Oliveira Batista Amauri Gouveia Karen R. H. Matos Oliveira Anderson Manoel Herculano Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress Frontiers in Behavioral Neuroscience anxiety acute stress CB1 receptors oxidative stress GABA zebrafish |
| author_facet |
Waldo Lucas Luz Mateus Santos-Silva Patrick Bruno Cardoso Nadyme Assad Edinaldo Rogério da Silva Moraes Alan Barroso Araújo Grisólia Danielle Valente Braga Luana Ketlen Reis Leão Suellen Alessandra Soares de Moraes Adelaide da Conceição Passos Evander de Jesus Oliveira Batista Evander de Jesus Oliveira Batista Amauri Gouveia Karen R. H. Matos Oliveira Anderson Manoel Herculano |
| author_sort |
Waldo Lucas Luz |
| title |
Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress |
| title_short |
Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress |
| title_full |
Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress |
| title_fullStr |
Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress |
| title_full_unstemmed |
Putative Activation of the CB1 Cannabinoid Receptors Prevents Anxiety-Like Behavior, Oxidative Stress, and GABA Decrease in the Brain of Zebrafish Submitted to Acute Restraint Stress |
| title_sort |
putative activation of the cb1 cannabinoid receptors prevents anxiety-like behavior, oxidative stress, and gaba decrease in the brain of zebrafish submitted to acute restraint stress |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Behavioral Neuroscience |
| issn |
1662-5153 |
| publishDate |
2021-01-01 |
| description |
Anxiety disorder is a well-recognized condition observed in subjects submitted to acute stress. Although the brain mechanisms underlying this disorder remain unclear, the available evidence indicates that oxidative stress and GABAergic dysfunction mediate the generation of stress-induced anxiety. Cannabinoids are known to be efficient modulators of behavior, given that the activation of the cannabinoid receptors type-1 (CB1 receptors) induces anxiolytic-like effects in animal models. In the present study, we aimed to describe the effects of the stimulation of the CB1 receptors on anxiety-like behavior, oxidative stress, and the GABA content of the brains of zebrafish submitted to acute restraint stress (ARS). The animals submitted to the ARS protocol presented evident anxiety-like behavior with increased lipid peroxidation in the brain tissue. The evaluation of the levels of GABA in the zebrafish telencephalon presented decreased levels of GABA in the ARS group in comparison with the control. Treatment with ACEA, a specific CB1 receptor agonist, prevented ARS-induced anxiety-like behavior and oxidative stress in the zebrafish brain. ACEA treatment also prevented a decrease in GABA in the telencephalon of the animals submitted to the ARS protocol. Overall, these preclinical data strongly suggest that the CB1 receptors represent a potential target for the development of the treatment of anxiety disorders elicited by acute stress. |
| topic |
anxiety acute stress CB1 receptors oxidative stress GABA zebrafish |
| url |
https://www.frontiersin.org/articles/10.3389/fnbeh.2020.598812/full |
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