Feeder-free generation and long-term culture of human induced pluripotent stem cells using pericellular matrix of decidua derived mesenchymal cells.

Feeder-free generation and long-term culture of human induced pluripotent stem cells using pericellular matrix of decidua derived mesenchymal cells.

Human ES cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are usually generated and maintained on living feeder cells like mouse embryonic fibroblasts or on a cell-free substrate like Matrigel. For clinical applications, a quality-controlled, xenobiotic-free culture system is required...

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Bibliographic Details
Main Authors: Hayato Fukusumi, Tomoko Shofuda, Daisuke Kanematsu, Atsuyo Yamamoto, Hiroshi Suemizu, Masato Nakamura, Mami Yamasaki, Masatoshi Ohgushi, Yoshiki Sasai, Yonehiro Kanemura
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3561375?pdf=render

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http://europepmc.org/articles/PMC3561375?pdf=render

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