A comparative analysis on serum antibody levels of sheep immunized with crude and thiol-purified excretory/secretory antigen of Haemonchus contortus
In vitro culture of live adult worms were made in RPMI 1640 medium at a concentration of approximately 50 worms / ml in a culture flask at 370 C for 24 hours and the culture supernatant was used as antigen. The E/S antigen was purified by thiol –sepharose affinity chromatography. On wes...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2012-10-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.scopemed.org/mnstemps/2/2-1327398879.pdf |