Mismatch amplification mutation assay-polymerase chain reaction: A method of detecting fluoroquinolone resistance mechanism in bacterial pathogens

Mismatch amplification mutation assay-polymerase chain reaction: A method of detecting fluoroquinolone resistance mechanism in bacterial pathogens

The mismatch amplification assay is a modified version of polymerase chain reaction (PCR) that permits specific amplification of gene sequences with single base pair change. The basis of the technique relies on primer designing. The single nucleotide mismatch at the 3' proximity of the reverse...

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Bibliographic Details
Main Authors: Vijaya Kumar Deekshit, Kadeeja Jazeela, Gunimala Chakraborty, Anusha Rohit, Anirban Chakraborty, Indrani Karunasagar
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2019-01-01
Series:Indian Journal of Medical Research
Subjects:
Fluoroquinolone resistance - mismatch amplification mutation assay - polymerase chain reaction - quinolone resistance determining region mutations
Online Access:http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2019;volume=149;issue=2;spage=146;epage=150;aulast=Deekshit

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http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2019;volume=149;issue=2;spage=146;epage=150;aulast=Deekshit

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