The extraction of inositol-containing phospholipids and phosphatidylcholine from Saccharomyces cerevisiae and Neurospora crassa.

By use of fungi grown in the presence of [3H]-inositol and [14C]choline, we have explored methods for the quantitative extraction of inositol-containing phospholipids and phosphatidylcholine. Slightly alkaline mixtures of both ethanol-water and ethanol-diethylether-water at elevated temperatures wer...

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Bibliographic Details
Main Authors: B A Hanson, R L Lester
Format: Article
Language:English
Published: Elsevier 1980-03-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520398102